High-throughput Analysis of DNA Modifying Enzymes by an Agilent RapidFire 360 System
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چکیده
Enzymes that act on DNA are promising targets for anticancer therapeutics1. For example, the epigenetic enzyme DNMT1 is inhibited by 5-azacytidine, causing DNA hypomethylation and enhancing the function of tumor suppressor genes2. In concordance, the development of accurate and user-friendly assays to study DNA modifications has become pivotal to drug discovery. Traditional in vitro assays have used radioactivity, antibodies, and fluorescent substrates to relate the extent of reaction. However, each of these approaches has drawbacks that limit its practicality for large sample numbers. This application note illustrates the advantages of developing high-throughput assays for DNA-modifying enzymes using an Agilent 360 RapidFire High-throughput Mass Spectrometry System. In particular, the abilities to detect native DNA directly and measure multiple DNA species in each reaction are highlighted. High-throughput Analysis of DNA Modifying Enzymes by an Agilent RapidFire 360 System
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